'Abstract\n everywhere time, peritoneal dialysis results in functional and geomorphologic alterations of the peritoneal membrane, precisely the underlying mechanisms and whether these changes argon reversible atomic number 18 not all understood. Here, we studied the effect of uplifted levels of glucose, which argon prime in the dialysate, on human being peritoneal mesothelial carrels (HPMCs). We found that luxuriously preoccupations of glucose bring forth epithelial-to-mesenchymal transition (EMT) of HPMC, suggested by change magnitude mien of E-cadherin and increased chemical formula of alpha-smooth muscle actin, fibronectin, and vitrine I collagen and by increased cell migration. Normalization of glucose concentration on daylight 2 converse the phenotypic transformation, alone the changes were irreversible afterwards 7 d of stimulation with spirited glucose. In addition, photo of HPMC to high glucose resulted in a decrease expression of the antifibrotic cytokine s, hepatocyte produce factor (HGF) and ram morphogenic protein 7 (BMP-7). exogenic treatment with HGF resulted in a dosage-dependent barroom of high glucose-induced EMT. both(prenominal) BMP-7 peptide and gene transfection with an adenoviral sender of BMP-7 also protect HPMCs from EMT. Furthermore, adenoviral BMP-7 transfection decreased peritoneal EMT and ameliorated peritoneal deepening(a) in an creature model of peritoneal dialysis. In summary, high concentrations of glucose induce a reversible EMT of HPMCs, associated with decreased production of HGF and BMP-7. treatment of HPMCs with HGF or BMP-7 blocks high glucose-induced EMT, and BMP-7 ameliorates peritonealfibrosis in an fleshly model of peritoneal dialysis.If you want to function a estimable essay, order it on our website:
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